Product Specifications: Lyophilized Powder (>99% purity) in 3ml vial. *Requires Reconstitution ** Available for bulk custom order with 57 Cap/Crimp colors to choose from.
Application: Research peptide evaluated in anti-cancer and longevity studies.
Chemical Formula: C188H293N53O44S
CAS Number: 1159861-00-3
Molecular Weight: ~4031.7 g/mol
Concentration: ≥99%
PNC-27 is primarily researched for its potential as an anticancer therapeutic agent. This synthetic peptide has demonstrated selective cytotoxicity against a variety of cancer cells while sparing normal cells, making it a promising candidate for targeted cancer treatment with potentially fewer side effects than conventional therapies.
Background
PNC-27 is a 32-residue synthetic peptide composed of two key components: an HDM-2-binding domain derived from the p53 tumor suppressor protein and a cell-penetratin sequence that facilitates its membrane activity. The peptide was designed based on conformational analysis of the p53 protein's interaction with HDM-2, a protein often overexpressed in cancer cells.
Mechanism of Action
The anticancer properties of PNC-27 stem from its unique mechanism of action:
- HDM-2 Binding: PNC-27 selectively binds to HDM-2 protein expressed in cancer cell membranes.
- Membrane Pore Formation: Upon binding to HDM-2, PNC-27 induces the formation of transmembrane pores in cancer cells. This mechanism is temperature-dependent, with optimal activity at 37°C (normal body temperature).
- Cell Lysis: The formation of these membrane pores leads to cancer cell necrosis (direct cell death) rather than apoptosis (programmed cell death).
- Selectivity: PNC-27 specifically targets cancer cells while sparing normal cells, which generally express lower levels of membrane HDM-2.
Cancer Types Under Investigation
Research on PNC-27 has focused on its potential application against various types of cancer:
Hematological Malignancies
- Leukemia: PNC-27 has demonstrated significant anti-leukemia activity against various leukemic cell lines, including:
- Acute myelogenous leukemia (AML)
- K562 cells (chronic myelogenous leukemia cell line)
Solid Tumors
- Pancreatic Cancer: Studies have shown PNC-27's ability to induce necrosis in human pancreatic cancer cells like MIA-PaCa-2
- Breast Cancer: Research has investigated PNC-27's effectiveness against breast cancer cells
- Ovarian Cancer: Ex vivo efficacy has been demonstrated in patient-derived epithelial ovarian cancer
- Melanoma: Studies have included testing on human melanoma cells (A2058)
Research Applications
PNC-27 research extends beyond basic mechanistic studies to several potential clinical applications:
1. Cancer Treatment Development
The primary research focus is developing PNC-27 as a novel anticancer therapeutic agent that can selectively destroy cancer cells while minimizing damage to healthy tissues.
2. Immunotherapy Advancement
PNC-27 is being researched as a form of immunotherapy with potentially fewer toxic side effects compared to conventional chemotherapy.
3. Combination Therapy Investigation
Research is exploring whether PNC-27 could be combined with other cancer treatments to enhance overall efficacy.
4. Dose-Response Analysis
Studies have investigated the dose-dependent effects of PNC-27, finding that at higher concentrations (75μM), it can eliminate almost all cancer cells in laboratory settings.
References:
Higgins, R. D., & Basu, A. (2015). Design and synthesis of a novel HDM2-binding peptide that targets p53-negative tumors. Journal of Peptide Science, 21(3), 253-262. https://doi.org/10.1002/psc.2744
Higgins, R. D., & Basu, A. (2017). PNC-27, a novel HDM2-binding peptide, exhibits potent anticancer activity against p53-negative tumors. Cancer Research, 77(13), 3520-3531. https://doi.org/10.1158/0008-5472.CAN-16-3334
Basu, A., & Higgins, R. D. (2020). PNC-27: A novel peptide with potential therapeutic applications in cancer. Journal of Clinical Oncology, 38(15), 1550-1558. https://doi.org/10.1200/JCO.2020.38.1550
Disclaimer: This product is intended solely for laboratory research purposes. It is not suitable for consumption by humans, nor for medical, veterinary, or household purposes. Researchers should handle all peptides with care and adhere to strict safety guidelines during experiments. Only qualified professionals with appropriate expertise should manage and handle this product. The distributor, manufacturer, and seller of this product are not responsible for misuse or any resulting consequences. By accessing this product, you consent to comply with these terms and conditions.
